diff --git a/pkgs/development/python-modules/pyopenssl/default.nix b/pkgs/development/python-modules/pyopenssl/default.nix
index f70aef65ec33..b6b36e82b520 100644
--- a/pkgs/development/python-modules/pyopenssl/default.nix
+++ b/pkgs/development/python-modules/pyopenssl/default.nix
@@ -16,14 +16,14 @@
 
 buildPythonPackage rec {
   pname = "pyopenssl";
-  version = "25.0.0";
+  version = "25.1.0";
   pyproject = true;
 
   src = fetchFromGitHub {
     owner = "pyca";
     repo = "pyopenssl";
     tag = version;
-    hash = "sha256-CQHLEtNb2jX7WNAYlmv5EIgepetMl81Xl3AJuRqOHow=";
+    hash = "sha256-QF511MVyjfddmkTd2H7RNJeoWs+e0me4i55YGP4t910=";
   };
 
   outputs = [